diff --git a/test/functional/abc_feature_proof_cleanup.py b/test/functional/abc_feature_proof_cleanup.py
--- a/test/functional/abc_feature_proof_cleanup.py
+++ b/test/functional/abc_feature_proof_cleanup.py
@@ -34,7 +34,6 @@
         self.num_nodes = 1
         self.extra_args = [[
             '-avalanche=1',
-            '-avaproofstakeutxodustthreshold=1000000',
             '-avaproofstakeutxoconfirmations=1',
             # Get rid of the getdata delay penalty for inbounds
             '-whitelist=noban@127.0.0.1',
diff --git a/test/functional/abc_p2p_avalanche_peer_discovery.py b/test/functional/abc_p2p_avalanche_peer_discovery.py
--- a/test/functional/abc_p2p_avalanche_peer_discovery.py
+++ b/test/functional/abc_p2p_avalanche_peer_discovery.py
@@ -52,7 +52,6 @@
         self.setup_clean_chain = True
         self.num_nodes = 1
         self.extra_args = [['-avalanche=1',
-                            '-avaproofstakeutxodustthreshold=1000000',
                             '-avaproofstakeutxoconfirmations=3']]
         self.supports_cli = False
 
diff --git a/test/functional/abc_p2p_avalanche_proof_voting.py b/test/functional/abc_p2p_avalanche_proof_voting.py
--- a/test/functional/abc_p2p_avalanche_proof_voting.py
+++ b/test/functional/abc_p2p_avalanche_proof_voting.py
@@ -39,7 +39,6 @@
         self.extra_args = [
             [
                 '-avalanche=1',
-                '-avaproofstakeutxodustthreshold=1000000',
                 '-avaproofstakeutxoconfirmations=2',
                 f'-avalancheconflictingproofcooldown={self.conflicting_proof_cooldown}',
                 f'-avalanchepeerreplacementcooldown={self.peer_replacement_cooldown}',
@@ -311,7 +310,6 @@
 
     def vote_tests(self, node):
         self.restart_node(0, extra_args=['-avalanche=1',
-                                         '-avaproofstakeutxodustthreshold=1000000',
                                          '-avaproofstakeutxoconfirmations=2',
                                          '-avacooldown=0',
                                          '-avalancheconflictingproofcooldown=0',
@@ -485,7 +483,6 @@
         # Restart the node with appropriate flags for this test
         self.restart_node(0, extra_args=[
             '-avalanche=1',
-            '-avaproofstakeutxodustthreshold=1000000',
             '-avaproofstakeutxoconfirmations=2',
             '-avalancheconflictingproofcooldown=0',
             '-avacooldown=0',
diff --git a/test/functional/abc_p2p_avalanche_quorum.py b/test/functional/abc_p2p_avalanche_quorum.py
--- a/test/functional/abc_p2p_avalanche_quorum.py
+++ b/test/functional/abc_p2p_avalanche_quorum.py
@@ -31,7 +31,6 @@
         self.min_avaproofs_node_count = 8
         self.extra_args = [[
             '-avalanche=1',
-            '-avaproofstakeutxodustthreshold=1000000',
             '-avaproofstakeutxoconfirmations=1',
             '-avacooldown=0',
             '-avatimeout=0',
diff --git a/test/functional/abc_p2p_avalanche_voting.py b/test/functional/abc_p2p_avalanche_voting.py
--- a/test/functional/abc_p2p_avalanche_voting.py
+++ b/test/functional/abc_p2p_avalanche_voting.py
@@ -25,7 +25,6 @@
         self.extra_args = [
             [
                 '-avalanche=1',
-                '-avaproofstakeutxodustthreshold=1000000',
                 '-avaproofstakeutxoconfirmations=1',
                 '-avacooldown=0',
                 '-avaminquorumstake=0',
diff --git a/test/functional/abc_p2p_compactproofs.py b/test/functional/abc_p2p_compactproofs.py
--- a/test/functional/abc_p2p_compactproofs.py
+++ b/test/functional/abc_p2p_compactproofs.py
@@ -54,7 +54,6 @@
         self.num_nodes = 2
         self.extra_args = [[
             '-avalanche=1',
-            '-avaproofstakeutxodustthreshold=1000000',
             '-avaproofstakeutxoconfirmations=1',
             '-avacooldown=0',
         ]] * self.num_nodes
diff --git a/test/functional/abc_p2p_getavaaddr.py b/test/functional/abc_p2p_getavaaddr.py
--- a/test/functional/abc_p2p_getavaaddr.py
+++ b/test/functional/abc_p2p_getavaaddr.py
@@ -87,7 +87,6 @@
         self.extra_args = [
             [
                 '-avalanche=1',
-                '-avaproofstakeutxodustthreshold=1000000',
                 '-avaproofstakeutxoconfirmations=1',
                 '-avacooldown=0',
                 '-avaminquorumstake=0',
diff --git a/test/functional/abc_p2p_proof_inventory.py b/test/functional/abc_p2p_proof_inventory.py
--- a/test/functional/abc_p2p_proof_inventory.py
+++ b/test/functional/abc_p2p_proof_inventory.py
@@ -54,7 +54,6 @@
         self.num_nodes = 5
         self.extra_args = [[
             '-avalanche=1',
-            '-avaproofstakeutxodustthreshold=1000000',
             '-avaproofstakeutxoconfirmations=2',
             '-avacooldown=0',
             '-whitelist=noban@127.0.0.1',
@@ -147,8 +146,7 @@
             }]
         )
 
-        self.restart_node(
-            0, ['-avalanche=1', '-avaproofstakeutxodustthreshold=1000000'])
+        self.restart_node(0, ['-avalanche=1'])
 
         peer = node.add_p2p_connection(P2PInterface())
         msg = msg_avaproof()
diff --git a/test/functional/abc_rpc_addavalanchenode.py b/test/functional/abc_rpc_addavalanchenode.py
--- a/test/functional/abc_rpc_addavalanchenode.py
+++ b/test/functional/abc_rpc_addavalanchenode.py
@@ -35,7 +35,6 @@
     def set_test_params(self):
         self.num_nodes = 1
         self.extra_args = [['-avalanche=1',
-                            '-avaproofstakeutxodustthreshold=1000000',
                             '-avaproofstakeutxoconfirmations=1',
                             '-avacooldown=0']]
 
diff --git a/test/functional/abc_rpc_avalancheproof.py b/test/functional/abc_rpc_avalancheproof.py
--- a/test/functional/abc_rpc_avalancheproof.py
+++ b/test/functional/abc_rpc_avalancheproof.py
@@ -58,8 +58,6 @@
         self.setup_clean_chain = True
         self.num_nodes = 2
         self.extra_args = [['-avalanche=1',
-                            '-avaproofstakeutxodustthreshold={}'.format(
-                                PROOF_DUST_THRESHOLD),
                             '-avaproofstakeutxoconfirmations=1',
                             '-avalancheconflictingproofcooldown=0',
                             '-avacooldown=0']] * self.num_nodes
diff --git a/test/functional/abc_rpc_getavalancheinfo.py b/test/functional/abc_rpc_getavalancheinfo.py
--- a/test/functional/abc_rpc_getavalancheinfo.py
+++ b/test/functional/abc_rpc_getavalancheinfo.py
@@ -33,7 +33,6 @@
             '-avacooldown=0',
             '-avaminquorumstake=250000000',
             '-avaminquorumconnectedstakeratio=0.9',
-            '-avaproofstakeutxodustthreshold=1000000',
             '-avaminavaproofsnodecount=0',
         ]]
 
diff --git a/test/functional/abc_rpc_getavalanchepeerinfo.py b/test/functional/abc_rpc_getavalanchepeerinfo.py
--- a/test/functional/abc_rpc_getavalanchepeerinfo.py
+++ b/test/functional/abc_rpc_getavalanchepeerinfo.py
@@ -25,7 +25,6 @@
         self.setup_clean_chain = True
         self.num_nodes = 1
         self.extra_args = [['-avalanche=1',
-                            '-avaproofstakeutxodustthreshold=1000000',
                             '-avaproofstakeutxoconfirmations=1',
                             '-avacooldown=0']]
 
diff --git a/test/functional/p2p_eviction.py b/test/functional/p2p_eviction.py
--- a/test/functional/p2p_eviction.py
+++ b/test/functional/p2p_eviction.py
@@ -66,7 +66,6 @@
         # avalanche availability score
         self.extra_args = [["-maxconnections=188",
                             "-avalanche=1",
-                            "-avaproofstakeutxodustthreshold=1000000",
                             "-avaproofstakeutxoconfirmations=1",
                             "-maxavalancheoutbound=16"]]
 
diff --git a/test/functional/p2p_inv_download.py b/test/functional/p2p_inv_download.py
--- a/test/functional/p2p_inv_download.py
+++ b/test/functional/p2p_inv_download.py
@@ -126,7 +126,6 @@
     def set_test_params(self):
         self.num_nodes = 2
         self.extra_args = [['-avalanche=1',
-                            '-avaproofstakeutxodustthreshold=1000000',
                             '-avaproofstakeutxoconfirmations=1',
                             '-avacooldown=0']] * self.num_nodes
 
diff --git a/test/functional/rpc_net.py b/test/functional/rpc_net.py
--- a/test/functional/rpc_net.py
+++ b/test/functional/rpc_net.py
@@ -46,11 +46,9 @@
         self.setup_clean_chain = True
         self.num_nodes = 2
         self.extra_args = [["-avalanche=1",
-                            "-avaproofstakeutxodustthreshold=1000000",
                             "-avaproofstakeutxoconfirmations=1",
                             "-minrelaytxfee=10"],
                            ["-avalanche=1",
-                            "-avaproofstakeutxodustthreshold=1000000",
                             "-avaproofstakeutxoconfirmations=1",
                             "-minrelaytxfee=5"]]
         self.supports_cli = False
diff --git a/test/functional/test_framework/test_node.py b/test/functional/test_framework/test_node.py
--- a/test/functional/test_framework/test_node.py
+++ b/test/functional/test_framework/test_node.py
@@ -115,6 +115,9 @@
             "-debugexclude=leveldb",
             "-uacomment=" + self.name,
             "-noprinttoconsole",
+            # Set the Avalanche stake dust threshold low so that tests do not
+            # need to combine coinbase UTXOs in order to stake.
+            "-avaproofstakeutxodustthreshold=1000000",
         ]
 
         if use_valgrind: