diff --git a/test/functional/chronik_serve.py b/test/functional/chronik_serve.py
--- a/test/functional/chronik_serve.py
+++ b/test/functional/chronik_serve.py
@@ -2,9 +2,11 @@
 # Distributed under the MIT software license, see the accompanying
 # file COPYING or http://www.opensource.org/licenses/mit-license.php.
 
+import os
+
 from test_framework.netutil import test_ipv6_local
 from test_framework.test_framework import BitcoinTestFramework
-from test_framework.util import assert_equal
+from test_framework.util import assert_equal, get_datadir_path
 
 
 class ChronikServeTest(BitcoinTestFramework):
@@ -30,6 +32,21 @@
 
         test_host("127.0.0.1", self.nodes[0].chronik_port)
 
+        datadir = get_datadir_path(self.options.tmpdir, 0)
+        config_path = os.path.join(datadir, "bitcoin.conf")
+        config_content = []
+        with open(config_path, "r", encoding="utf8") as f:
+            config_content = f.readlines()
+
+        os.unlink(config_path)
+
+        with open(config_path, "w", encoding="utf8") as f:
+            [
+                f.write(line)
+                for line in config_content
+                if not line.startswith("chronikbind")
+            ]
+
         self.restart_node(0, ["-chronik", "-chronikbind=0.0.0.0"])
         test_host("127.0.0.1", 18442)