diff --git a/test/functional/mempool_reorg.py b/test/functional/mempool_reorg.py
--- a/test/functional/mempool_reorg.py
+++ b/test/functional/mempool_reorg.py
@@ -32,7 +32,6 @@
         self.log.info("Add 4 coinbase utxos to the miniwallet")
         # Block 76 contains the first spendable coinbase txs.
         first_block = 76
-        wallet.rescan_utxos()
 
         # Three scenarios for re-orging coinbase spends in the memory pool:
         # 1. Direct coinbase spend  :  spend_1
diff --git a/test/functional/mempool_spend_coinbase.py b/test/functional/mempool_spend_coinbase.py
--- a/test/functional/mempool_spend_coinbase.py
+++ b/test/functional/mempool_spend_coinbase.py
@@ -28,7 +28,6 @@
         chain_height = 198
         self.nodes[0].invalidateblock(self.nodes[0].getblockhash(chain_height + 1))
         assert_equal(chain_height, self.nodes[0].getblockcount())
-        wallet.rescan_utxos()
 
         # Coinbase at height chain_height-100+1 ok in mempool, should
         # get mined. Coinbase at height chain_height-100+2 is
diff --git a/test/functional/p2p_blocksonly.py b/test/functional/p2p_blocksonly.py
--- a/test/functional/p2p_blocksonly.py
+++ b/test/functional/p2p_blocksonly.py
@@ -19,9 +19,6 @@
 
     def run_test(self):
         self.miniwallet = MiniWallet(self.nodes[0])
-        # Add enough mature utxos to the wallet, so that all txs spend
-        # confirmed coins
-        self.miniwallet.rescan_utxos()
 
         self.blocksonly_mode_tests()
         self.blocks_relay_conn_tests()
diff --git a/test/functional/p2p_filter.py b/test/functional/p2p_filter.py
--- a/test/functional/p2p_filter.py
+++ b/test/functional/p2p_filter.py
@@ -281,7 +281,6 @@
 
     def run_test(self):
         self.wallet = MiniWallet(self.nodes[0])
-        self.wallet.rescan_utxos()
 
         filter_peer = self.nodes[0].add_p2p_connection(P2PBloomFilter())
         self.log.info("Test filter size limits")
diff --git a/test/functional/rpc_blockchain.py b/test/functional/rpc_blockchain.py
--- a/test/functional/rpc_blockchain.py
+++ b/test/functional/rpc_blockchain.py
@@ -516,7 +516,6 @@
         node = self.nodes[0]
 
         miniwallet = MiniWallet(node)
-        miniwallet.rescan_utxos()
 
         fee_per_byte = Decimal("0.1")
         fee_per_kb = 1000 * fee_per_byte
diff --git a/test/functional/rpc_scantxoutset.py b/test/functional/rpc_scantxoutset.py
--- a/test/functional/rpc_scantxoutset.py
+++ b/test/functional/rpc_scantxoutset.py
@@ -28,7 +28,6 @@
 
     def run_test(self):
         self.wallet = MiniWallet(self.nodes[0])
-        self.wallet.rescan_utxos()
 
         self.log.info("Create UTXOs...")
         pubk, spk, addr = getnewdestination()
diff --git a/test/functional/setup_scripts/chronik-client_blocktxs_and_tx_and_rawtx.py b/test/functional/setup_scripts/chronik-client_blocktxs_and_tx_and_rawtx.py
--- a/test/functional/setup_scripts/chronik-client_blocktxs_and_tx_and_rawtx.py
+++ b/test/functional/setup_scripts/chronik-client_blocktxs_and_tx_and_rawtx.py
@@ -25,7 +25,6 @@
         # Init
         node = self.nodes[0]
         wallet = MiniWallet(node)
-        wallet.rescan_utxos()
 
         yield True
 
diff --git a/test/functional/test_framework/wallet.py b/test/functional/test_framework/wallet.py
--- a/test/functional/test_framework/wallet.py
+++ b/test/functional/test_framework/wallet.py
@@ -51,6 +51,13 @@
             self._test_node.validateaddress(self._address)["scriptPubKey"]
         )
 
+        # When the pre-mined test framework chain is used, it contains coinbase
+        # outputs to the MiniWallet's default address in blocks 76-100
+        # (see method BitcoinTestFramework._initialize_chain())
+        # The MiniWallet needs to rescan_utxos() in order to account
+        # for those mature UTXOs, so that all txs spend confirmed coins
+        self.rescan_utxos()
+
     def _create_utxo(self, *, txid, vout, value, height):
         return {"txid": txid, "vout": vout, "value": value, "height": height}