diff --git a/test/functional/test_framework/test_framework.py b/test/functional/test_framework/test_framework.py
--- a/test/functional/test_framework/test_framework.py
+++ b/test/functional/test_framework/test_framework.py
@@ -298,6 +298,8 @@
             rpc_logger.removeHandler(h)
         if cleanup_tree_on_exit:
             shutil.rmtree(self.options.tmpdir)
+
+        self.nodes.clear()
         return exit_code
 
     # Methods to override in subclass test scripts.